Bacopa

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(previous URL: http://wakandb.u-toyama.ac.jp/wiki/LCMS:Bacopa_INM-484)

試料情報

Voucher No. INM-484

ダウンロード

• MSBデータ (Bacopa エキス)
• MSBデータ (Bacoside A2 標準品)
• MSBデータ (Bacopaside I 標準品)

TIC, MC, and Compounds

Analytical conditions 1 (Fig. 1)

Instrument	Shimadzu LC-IT-TOF MS ESI
Column	Waters Atlantis T3 (2.1 mm x 150 mm)
Column Temperature	40 °C
Solvent A	5 mM Ammonium acetate solution
Solvent B	acetonitrile
Gradient	10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage	4.5 kV (Positive ion mode), -3.5 kV (Negative ion mode)
Capillary temperature	200 °C
Nebulizer gas	1.5 l/min

Analytical conditions 2 (Fig. 2)

Instrument	TSQ7000 ESI
Column	Waters Atlantis T3 (2.1 mm x 100 mm, 3 μm)
Column Temperature	40 °C
Solvent A	5 mM Ammonium acetate solution
Solvent B	acetonitrile
Flow rate:	0.15 ml/min
Gradient	10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage	-3.5 kV (Negative ion mode)

Analytical conditions 3 (Figs. 3-5)

Instrument	TSQ7000 ESI
Column	Waters Atlantis T3 (2.1 mm x 100 mm, 3 μm)
Column Temperature	40 °C
Solvent A	0.1% HCOOH
Solvent B	acetonitrile
Flow rate:	0.15 ml/min
Gradient	10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage	-3.5 kV (Negative ion mode)

Remarks

Figs. 2 and 3 show effect of chromatographic conditions on separation of bacopasides. When CH₃COONH₄ was used as solvent A (Fig. 2), the order of elution was: Bacopaside I → Bacoside A₃ → Bacoside A₂ → Bacopaside N₁ → Bacopaside N₂. In contrast, when HCOOH was used as solvent A (Fig. 3), the order of elution was: Bacoside A₃ → Bacoside A₂ → Bacopaside N₁ → Bacopaside N₂ → Bacopaside I. Figs. 4 and 5 show Quantitation of bacopasides. Fig. 4: Standard compounds. Fig. 5: Extract (10 mg/ml solution, 1 μl injection).

このデータを引用している論文

• Hironori Fujiwara, Ryohei Tsushima, Ryo Okada, Suresh Awale, Ryota Araki, Takeshi Yabe, Kinzo Matsumoto: Sansoninto, a traditional herbal medicine, ameliorates behavioral abnormalities and down-regulation of early growth response-1 expression in mice exposed to social isolation stress, J. Tradit. Complement. Med., 8(1):81-88 (2017).
  https://doi.org/10.1016/j.jtcme.2017.03.004

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